Our teams are in regular contact with suppliers and distributors worldwide to manage inventory of raw materials to ensure continued availability. Nanoglo dualluciferase reporter assay protocol promega. Due to the covalent linkage, halotag fusion proteins cannot be eluted from the halolink resin, which allows extensive washing to remove nonspecifically bound proteins without the danger of eluting halotag fusion proteins. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 phone 6082744330 fax 6082772516 printed in usa. Instructions for use of products dc2705, dc2720 and dc2780. Promega products are warranted to meet or exceed the stated specifications. Controlled versions of department of forensic biology manuals only exist in the. Promega products contain chemicals which may be harmful if misused. Dualluciferase reporter assay system harvard university. The reagent is prepared by mixing nanoglo luciferase assay substrate and nanoglo luciferase assay buffer and contains an integral lysis buffer to use directly on cells expressing nanoluc luciferase or culture medium when the luciferase. The broad array of spectramax i3x detection cartridges enables the user.
Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 1 tm426 revised 615. This manual contains protocols for use of the powerplex fusion 6c system with the geneamp pcr system 9. Protocols for dna amplification using the powerplex 21 system. Buccal cotton swab samples were incubated in swabsolution reagent promega corporation as directed in the technical manual 7, and 2. Yfiler plus pcr amplification kit thermo fisher scientific.
Brightglo luciferase assay system protocol promega. The two genes encoding the two potentially interactive proteins of interest are cloned into pbind and pact vectors to generate fusion proteins with the dnabinding domain of gal4 and the activation domain of. View and download generalmusic promega 2 owners manual online. Manufactured and qualitycontrolled at new england biolabs, thermo scientific phusion highfidelity dna polymerase offers both high fidelity and robust performance, and thus can be used for all pcr applications. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. The information in this guide is subject to change without notice. Size standard deviation for each allele within the powerplex fusion allelic ladder mix. Use of the powerplexr fusion 6c system to amplify dna. The powerplex fusion system is compatible with the abi prism 3100 and 3100avant.
Prepfiler express tm and prepfiler express bta tm forensic dna extraction kits user guide. Promega, and qiagen for providing the kits used in this study. Dualglo luciferase assay system technical manual promega. The powerplex fusion system is compatible with the abi prism 3100 and 3100avant genetic analyzers and applied biosystems 3, 3xl, 3500 and 3500xl genetic analyzers. Brightglo luciferase assay system technical manual promega. Spectrum compact ce system promega france pdf catalogs. Please contact promega technical services if you have questions on use of this system.
Materials to be supplied by the user microamp optical 96well reaction plate applied. The powerplex fusion 6c system is compatible with the applied biosystems 3500 genetic analyzer as reported in the technical manual 5. This protocol was taken from the technical manual of the wizard genomic dna purification kit, and modified on july th, 2005. Use of the powerplex fusion 6c system to amplify extracted dna. For users setting up their quad head for the first time and looking to gain a deep understanding of the system to get the best results, start here stepsmm. Powerplex fusion system technical manual promega corporation.
Instructions for use of products e2920, e2940, e2980. Product components and storage conditions productsizecat. Use of the powerplexr fusion system to amplify dna from swabs. To deal with the increase in information and sensitivity gained from a 24plex str amplification kit, the richland county sheriffs departments dna laboratory evaluated and validated a new data analysis software, genemarker hid v 2.
For users setting up quadfusion heads on their own system, important information can be found here this user manual is a work in progress and may contain incomplete or incorrect information. Erie county forensic laboratory strmix validationfusion page 1 of 32 1 strmix implementation and internal validationfusion 2 erie county central police services forensic laboratory 3 inhouse studies were undertaken to configure, implement and validate strmix software v2. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Find the best resources for 3d printing with simplify3d including videos, tutorials, print quality tips, faqs and more. Visit the web site to verify that you are using the most current version of this technical manual.
If you are a more experienced 3d printer user, get. In addition to the loci listed above, the globalfiler iqc pcr amplification kit amplifies 2 internal quality control iqc markers. Each promega product is shipped with documentation stating specifications and other technical information. The powerplex fusion system allows coamplification and fluorescent detection of 24 loci, including the core codis loci. Dna technical leader powerplex fusion sample preparation for. The yfiler plus pcr amplification kit uses the same improved process for synthesis and purification of the amplification primers that are previously developed for other nextgeneration thermo fisher scientific str chemistries. Excerpts and links may be used, provided that full and clear credit is given to promega corporation with appropriate and specific direction to the original content. Protocol for processing buccal swabs materials to be supplied by the user clickfit microtube, 1. Promega powerplex fusion 5dye cc5 internal lane standard 500 fga a vwa d18s51 d8s1179 d19s433 d21s11 d3s58 th01 d16s539 d2s38 d22s1045 d2s441 penta e 100 bp 200 bp 300 bp 400 bp d1s1656 d12s391 d10s1248 d5s818 ds317 d7s820 csf1po tpox dys391 24plex fl joe tmret cxret penta d. Manual polyhistidine or hqtagged protein purification 6 e. In the flp in system, three different vectors are used to generate isogenic stable mammalian cells lines expressing your genes of interest. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 3 tm426 revised 615 2. Summary of changes the following changes were made to the 417 revision of this document. Due care should be exercised with all promega products to prevent direct human contact.
If you are a complete beginner to 3d printing, please start at the beginner guides. The promega is expandable, meaning you can customize it to suit your needs. Hence, we compared the efficiency of a beta test version of promegas bone demineralization buffer vs. Dna technical leader powerplex fusion sample preparation. Once amplified, samples were separated on an applied biosystems 3 genetic analyzer using 3 kv, 5second injection. Moreover, we reduced the starting bone powder from 200 mg to 25 mg to determine effectiveness of the demineralization. The first major component of the flp in system is the pfrtlaczeo target site vector that is used to generate a flpin host cell line. Email promega technical services if you have questions on use of this system.
The nanoglo dualluciferase reporter nanodlr assay system allows sensitive detection of firefly and nanoluc luciferases. Powerplex esx fast and esi fast powerplex fusion 6c. The primer set and the allelic ladder in the globalfiler kit are different. Amplification kits globalfiler and globalfiler iqc pcr. Forensic biology protocols for forensic str analysis manual.
Powerplex fusion 6c system for use on the applied biosystems. This reagent is not required for the powerplex fusion 6c, powerplex fusion, powerplex 18d, powerplex 21 and powerplex y23 systems. Internal validation of the promega powerplex fusion amplification. Checkmate mammalian twohybrid system protocol promega. The maxwell rsc rna ffpe kit is used with the maxwell rsc instrument to purifiy rna from formalinfixed, paraffinembedded ffpe mammalian tissue samples. The powerplex fusion 6c system is a 6color, 27locus multiplex for human. The spectrum compact control software provides a simple user interface with a clear display of useful features including run set up, consumables and array usage information, as well as system maintenance reminders. Powerplex fusion promega powerplex y23 promega applied genetics background on str loci and kits. Promega powerplex fusion 6c system receives approval by.
If configuring in a windows workgroup, all computers running dna fusion must be members of single dedicated workgroup. In addition, the pbind vector expresses the renilla reniformis luciferase, which allows the user to normalize the transfection efficiency. Maker of the original micro, the first consumer 3d printer to deliver great quality 3d printing in a compact, affordable package. This revolution in modularity makes nocompromise detection the norm. If autoanalysis of sample data is desired, refer to the instrument users manual for instructions. Flexi rabbit reticulocyte lysate system technical manual. Several approaches to this technique have been published, but these methods generally require singlestranded dna. Its unique structure, a novel pyrococcuslike enzyme. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Open options strongly recommends that all computers running dna fusion are connected on a windows domain. The dualglo luciferase assay system is designed to allow highthroughput analysis of mammalian cells containing genes for firefly and renilla luciferases, grown in 96 or 384well plates. Use of the powerplexr fusion system to amplify extracted dna.
Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Str loci and multiplex kits becky hill research biologist, applied genetics group. Business wirethe promega powerplex fusion 6c system was granted approval by the fbi for use in laboratories that generate dna records for the national dna index system ndis. An assay is required for all named samples on a plate. Materials to be supplied by the user clickfit microtube, 1. Nanoglo luciferase assay system technical manual promega. Dna fusion may be ran across different windows domains provided a bidirection trust is in place between domains.
Globalfiler and globalfiler iqc pcr amplification kits user guide for use with. One microliter of allelic ladder was detected using a applied biosystems 3500xl genetic analyzer at promega corporation using a 1. Promega official m3d promega repository for tracking sd card configs, slicer configs, and issues votingtracking smt 27 26 2 1 updated. Buccal cotton swab samples were incubated in swabsolution reagent promega corporation as directed in the technical manual, and 2. The two genes encoding the two potentially interactive proteins of interest are cloned into pbind and pact vectors to generate fusion proteins with the dnabinding domain of gal4 and the. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 phone 6082744330 fax 6082772516. Nanoglo luciferase assay system provides a simple, singleaddition reagent that generates a glowtype signal in the presence of nanoluc luciferase. Forensic biology protocols for forensic str analysisamplification using the power plex fusion system status. The spectramax i3x multimode detection platform utilizes a patentpending design that allows for realtime system configuration by the user in less than two minutes. For more information on the iqc markers, see chapter 5, assess the pcr reaction with the internal quality control system. Please exercise caution with the information contained herein. This user manual is a work in progress and may contain incomplete or incorrect information.
Rna can be extracted from up to 16 preprocessed samples in less than 60 minutes and can be used directly in a variety of downstream applications, including rtpcr. Flexi rabbit reticulocyte lysate system technical manual tb127. Dualluciferase reporter assay system protocol promega. Promega powerplex fusion 6c system receives approval by the. Yfiler plus pcr amplification kit user guide catalog numbers 4484678 and 4482730 publication number 4485610 revision c. Erie county central police services forensic laboratory. Powerplex fusion sample preparation for amplification nyc. Reagent as recommended in the swabsolution kit technical manual. Erie county forensic laboratory strmix validation fusion page 1 of 32 1 strmix implementation and internal validation fusion 2 erie county central police services forensic laboratory 3 inhouse studies were undertaken to configure, implement and validate strmix software v2. Manual polyhistidine or hqtagged protein purification 1. Use of the powerplexr fusion 6c system to amplify dna from. To 1ml of culture, add 100l of the fastbreak reagentdnase i. Refer to the applied biosystems 35003500xl genetic analyzer user guide for the instrument maintenance schedule and instructions to install the capillary array.
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